UK Products and Services - Methods and Techniques

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Assay of Protein Concentration

Different methods of determining protein concentration, as a resource for researchers and students in biochemistry and molecular biology laboratories from University of Nebraska, Lincoln, NE.
(http://www-class.unl.edu/biochem/protein_assay/)

Basic Principles of Gene Cloning, Sequencing, & Analysis

Description of methods behind gene cloning, genetic engineering, DNA recombination and isolation, including discussion of restriction enzymes and ligase.
(http://www.geocities.com:80/SiliconValley/5504/biochem.html)

BBHelp

Collection of molecular biology techniques derived from help files of Dr. Harold Zappe at the Department of Medical Microbiology, University of Cape Town, South Africa.
(http://www.uct.ac.za/depts/mmi/bbhelp/intro.html)

Beginning Laboratory Manual

Teaching manual to support the applied biotechnology programs at the University of Maryland, Baltimore.
(http://www.research.umbc.edu/~jwolf/method1.html)

Biomedpage

Collection of laboratory protocols, applications and links to publications in biomedicine and molecular biology from author in Germany.
(http://www.biomedpage.de/indexe.html)

Biopolymer Calculator

Calculates molecular weight, composition and concentration of nucleic acids and proteins, based on provided sequence, dilution and absorbance measurement. Also melting temperatures for nucleic acids. Maintained at Schepartz Laboratory of Chemical Biology, Yale, New Haven, CT.
(http://paris.chem.yale.edu/extinct.html)

BioProtocol

Internet project initiated in early 1992 as BRNI, bio.com includes PCR, hybridization and microarray protocols.
(http://bioprotocol.bio.com/protocolstools/)

biowww.net: Molecular Biology

Comprehensive trouble shooting archive, with protocol and newsgroup links, including cell biology, immunology and genetics.
(http://www.biowww.net/index.php/article/archive/9/)

Clontechniques

Quarterly newsletter, with search to access new product information and applications research updates and earlier articles from of Clontech, now in the BD BioSciences Group, at Palo Alto, CA.
(http://www.clontech.com/archive/index.shtml)

Comet Assay Interest Group

Forum for exchange of views on the single cell gel electrophoresis system, capable of detecting single- and double-strand breaks, oxidative DNA base damage, and DNA, protein and drug crosslinking. Includes related links.
(http://cometassay.com)

Comparative Genomic Hybridization

CGH is a molecular cytogenetic method of screening a tumor for genetic changes. CGH protocols, images, publications, events and links maintained by the University Hospital Charité, Humboldt-University of Berlin, Germany.
(http://amba.charite.de/cgh/)

Comprehensive Protocol Collection

Comprehensive collection of molecular biology and Nematode-related protocols, including many standard biological assays & stainings.
(http://www.dartmouth.edu/artsci/bio/ambros/protocols.html)

ecrBrowser

Evolutionary conserved regions comparison of the whole genome scale alignment of human, mouse and fugu genomes at LBNL, Berkley, CA.
(http://nemo.lbl.gov/ecrBrowser/)

Hirsh Lab Gel Documentation System

Description of an inexpensive agarose gel set-up. Includes instructions for camera operation with a script and then computer side image analysis.
(http://www.people.virginia.edu/~jb6vz/gel/)

Home Page of Andy Vierstraete

The use of DNA techniques in phylogenesis. Explanations, protocols and animations from Andy Vierstraete at the University of Ghent, Belgium.
(http://allserv.ugent.be/~avierstr/)

iProtocol for Bioscience

Key protocols used in biochemistry, bioengineering, cytobiology, genetics and molecular biology at MIT, Cambridge, MA.
(http://iprotocol.mit.edu)

Laboratory Dialysis Answers

Discussion board for laboratory dialysis tips and techniques, moderated by Spectrum Laboratories, Inc.
(http://www.labdialysischat.com/)

Molbiol.net

Resource for molecular biologists on methods, with feedback for user-directed evolution and links to commercial suppliers from Jonathan D Rees, Oxford, UK.
(http://www.molbiol.net/)

MolBio-Spot

Richard Man-kit Yu's site where you can acquire various molecular biology techniques.
(http://www.geocities.com/richyuhk/)

Molecular Biology Protocols

Resources such as polymerase chain reaction, dedicated to method development. Part of internet directory maintained by Horizon Scientific Press, Wymondham, UK.
(http://highveld.com/protocols.html)

Molecular Biology Protocols

Protocol collection, with interactive forum for over ten topics, covering DNA, PCR, RNA and proteins techniques and links to online journals and bioinformatics resources, from the Northwest Fisheries Science Center Microbial Pathogenesis Lab, Seattle, WA.
(http://micro.nwfsc.noaa.gov/protocols/)

Molecular Cloning Manual

Subscription only source of laboratory techniques in DNA and protein science, to complement the hard copy, giving scientists the benefit of both media from CSHL, NY.
(http://www.molecularcloning.com/)

Molecular Probes, Inc.

Handbook of fluorescence applications, bibliography, data tables and calculators for molecular biology, information on courses and workshops from supplier in Eugene, OR and Leiden, Netherlands.
(http://www.probes.com/resources/)

Patrick Beisser's Web Site

This site provides links to various sites relevant for molecular biology and bioinformatics. It also contains personal pages such as photographs and recreational pages.
(http://mapage.noos.fr/beisser/index.html)

Practical Molecular Biology

Methods, information, programs and BioMail for molecular biologists, as joint venture between Germany and Russia.
(http://molbiol.ru/eng/)

Recombinant DNA

Preparing probe, cloning into plasmids, complementarity and hybridization, gel electrophoresis, transfer to solid support and blotting variants, blocking, target detection PCR problems from chapter in MIT hypertext handbook.
(http://web.mit.edu/esgbio/www/rdna/rdnadir.html)

Reto's Buffers and Media Book

Alphabetical list for buffer recipes, including constituents for cultures, from personal page in Switzerland.
(http://www.ikp.unibe.ch/~rportman/buffersmedia.html)

Richard Owczarzy's Space

Online calculations of DNA extinction coefficients, melting temperatures, and links to molecular biology databases from commercial scientist in Coralville, IA.
(http://www.owczarzy.net/)

SciWeb Protocols and Reagents

Directory of molecular biology protocol links.
(http://sciweb.com/features/protocols.cfm)

SNP Scoring

Overview of genotyping, detection and quantifying single nucleotide polymorphism, including the application of template directed dye terminator incorporation assay. Offers database and links to other resources from PerkinElmer Life Sciences, Boston, MA.
(http://lifesciences.perkinelmer.com/areas/snps/)

Surgery Research

Collection of DNA microarray technology protocols, primarily for resident students at Stanford.
(http://surgeryresearch.com/)

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